background the physiological link between ghrelin and growth hormone (gh) has not yet been fully clarified. furthermore, the existence of a negative feedback mechanism between growth hormone–insulin-like growth factor (gh–igf)-i axis and ghrelin and the influence of amino acids on ghrelin secretion in children remain matters of debate. objectives to understand the regulation of ghrelin secretio...

in this article, we present the case of a 12-year-old female child who complained of bilateral temporal and frontal headache for 2 to 3 months with nausea and vomiting. physical examination revealed right-sided sixth cranial nerve palsy and papilledema in ophthalmoscopy. to find the cause of increased intracranial pressure, the patient underwent brain imaging and brain mri showed no abnormality...

The effects of growth hormone in the whole animal have been recently reviewed (1, 2). The following points are pertinent to the present work. The first injection of growth hormone into the hypophysectomized animal induces a hypoglycemic reaction similar to that which follows a small dose of insulin. This reaction is much less marked in a normal animal. After repeated injections of the hormone o...

human growth hormone (hgh) genomic sequence containing 5 exons and 4 introns was cloned in pcdna-3 and the constructed plasmid was subsequently used for transfection ofnlli-3t3 cell line using lipofection technique. expression of hgh in stably transfected cells was assayed using elisa. total rna was extracted from transfected cells and hgh cdna was amplified by rt-pcr using specific primers. th...

in this study escherichia coli de3 containing expression vector (pet21a) with cloned persian sturgeon growth hormone (psgh) gene was grown in 10 ml lb broth on a 150 rpm shaker, at the temperature of 37 °c. at the late log phase (determined by od standard curve) 100 μl isopropyl β-d-1-thiogalactopyranoside (iptg) was added for induction of gh synthesis. samples were taken every 2 hours and afte...